PSCAE gene, UPII gene, and E1A gene express in the recombinant adenovirus were identified by PCR. Firstly, harvested viruses were digested by proteinase K (Takara Biotechnology Co., Dalian, China), and then extracted virus DNA. PCR were performed according to PCR Reaction Kit (Takara) instruction. Gene expression bands were observed by agarose gel electrophoresis. The primer sequences were listed in Table 1 [9 (link), 18 (link)].

The primers used for polymerase chain reaction (PCR)

PCR primersPrimers sequence
PSCAEForward: 5′ GCTGACCGGTAGAGGCCAGCAGCACCCCTG 3′Reverse: 5′ CGGTGCTAGCAACTGCTTCCGTGTGTGGCTGACAG 3’
UPIIForward: 5’ ACT TTG AGC CTA CCC TTC C 3′Reverse: 5′ CAG TGA GCC GAG ATT GTG 3’
E1AForward: 5’ CAT GCC ACA GGT CCT CAT ATA GC 3′Reverse: 5′ GAG ACA TAT TAT CTG CCA CGG AGG 3’

PSCAE prostate stem cell antigen enhancer, UPII uroplakin II promoter, E1A the early adenoviral genes

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