Molecular Weight and Central Delivery

All animal procedures were conducted in accordance with Radboud University Medical Center animal welfare guidelines. Nine male Sprague–Dawley rats with an average weight of 250 g were anesthetized with 2.5–3 % isoflurane inhalation anesthesia and injected with 150 μl of I-125–labeled cetuximab (146 kDa), etanercept (51 kDa), and anakinra (17 kDa). Drugs with different molecular weights were selected to determine the effect of molecular weight on central delivery. For each drug, two rats were given injections in the area overlying the cervical spine at the C6-C7 level using a 30-gauge needle at a depth of 6 mm as described by Tobinick et al. [16 (link)]. One rat was given an injection in the dorsal tail vein, followed by flushing with 1 ml of saline. Directly after injection, the rats were placed in head-down position for 3 minutes. Five minutes after the injection, single-photon emission computed tomography (SPECT) was performed using a U-SPECT-II/CT scanner (MILabs, Utrecht, the Netherlands). After completion of scanning, which took 20 minutes, all rats were euthanized and bio-distribution was determined.

Free full text: Click here

Roerink M.E., Groen R.J., Franssen G., Lemmers-van de Weem B., Boerman O.C, & van der Meer J.W. (2015). Central delivery of iodine-125–labeled cetuximab, etanercept and anakinra after perispinal injection in rats: possible implications for treating Alzheimer’s disease. Alzheimer's Research & Therapy, 7, 70.

Publication 2015

U-SPECT-II/CT scanner

Anakinra Animal Cervical spine Cetuximab Ct scanner Delivery Drug Etanercept Head I 125 Inhalation anesthesia Isoflurane Male Needle Rats Saline Single photon emission computed tomography Sprague dawley rats Tail Vein

Corresponding Organization :

Other organizations : Radboud University Nijmegen, Radboud University Medical Center, University Medical Center Groningen, University of Groningen

Top 5 similar protocols

Variable analysis

independent variables

Molecular weight of injected drugs (cetuximab, etanercept, anakinra)

dependent variables

Bio-distribution of the injected drugs

control variables

Animal species (Sprague-Dawley rats)
Animal sex (male)
Average animal weight (250 g)
Anesthesia (2.5-3% isoflurane inhalation)
Injection site (area overlying the cervical spine at the C6-C7 level, dorsal tail vein)
Injection volume (150 μl)
Post-injection head-down position (3 minutes)
Time between injection and SPECT scanning (5 minutes)
SPECT scanning duration (20 minutes)

controls

Positive control: Injection in the dorsal tail vein, followed by flushing with 1 ml of saline

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!