Isolation of Mononuclear Cells from PBL
Corresponding Organization :
Other organizations : Emory University, Georgia Institute of Technology, University of Alabama at Birmingham
Variable analysis
- Mononuclear cell isolation method (ficoll density gradient centrifugation)
- Tissue mincing method (scalpel)
- Enzyme treatment (Liberase TL 125 ug/mL, DNAse I 100 ug/mL)
- Incubation time (45 min)
- Vortexing frequency (every 10 min)
- Isolated mononuclear cells
- Cell suspension after tissue digestion
- DMEM medium
- Antibiotic/antimycotic (GIBCO)
- Sterile 70 um mesh for filtration
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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