Millifluidic Lineage Tracking Assay

The analysis with the millifluidic lineage tracking machine was performed after dilution in fresh LB medium (Sigma-Aldrich) to obtain, an average number of cell per drop λ ≈ 0.5. Drops were incubated and measured at 37°C as described in [12 (link), 13 (link)]. The fluorescence was measured using a photomultiplier (Hamamatsu) and a YFP filter set (479/40nm excitation and 530/40nm emission, Thorlabs) and an LED source (490nm, Thorlabs). In order to compare experiments, the data were normalized according to a reference measurement on the ancestral population.

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Cottinet D., Condamine F., Bremond N., Griffiths A.D., Rainey P.B., de Visser J.A., Baudry J, & Bibette J. (2016). Lineage Tracking for Probing Heritable Phenotypes at Single-Cell Resolution. PLoS ONE, 11(4), e0152395.

Publication 2016

photomultiplier

Dilution Fluorescence

Corresponding Organization : Wageningen University & Research

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Variable analysis

independent variables

Dilution of the sample in fresh LB medium (Sigma-Aldrich) to obtain an average number of cells per drop λ ≈ 0.5

dependent variables

Fluorescence measurements using a photomultiplier (Hamamatsu) and a YFP filter set (479/40nm excitation and 530/40nm emission, Thorlabs) and an LED source (490nm, Thorlabs)

control variables

Incubation and measurement temperature at 37°C
Normalization of the data to a reference measurement on the ancestral population

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