Transmission Electron Microscopy of Tellurium Nanostructures

Te-nanostructure extracts were recovered from BCP1 resting cells following the procedure published in our previous study^{21 (link)}, while Transmission Electron Microscopy (TEM) imaging of both TeO₃²⁻-exposed BCP1 resting cells and Te-nanostructure extracts was performed using a Hitachi H7650 TEM. For bright field (BF) and high-resolution (HR) TEM, as well as the corresponding Selected-Area Electron Diffraction (SAED) pattern of TeNRs were collected by FEI Tecnai F20 TEM at an acceleration voltage of 200 kV. The samples were prepared by mounting 5 µL of either cellular suspensions or Te-nanostructure extracts on carbon-coated copper grids (CF300-CU, Electron Microscopy Sciences), which were air-dried prior the imaging. TEM micrographs were analyzed through ImageJ software to measure the actual length of TeNRs, which was calculated considering 100 randomly chosen nanorods. The distribution was fitted to a Gaussian function to yield the average length of TeNRs.

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Presentato A., Piacenza E., Darbandi A., Anikovskiy M., Cappelletti M., Zannoni D, & Turner R.J. (2018). Assembly, growth and conductive properties of tellurium nanorods produced by Rhodococcus aetherivorans BCP1. Scientific Reports, 8, 3923.

Publication 2018

H7650 TEM Tecnai F20 TEM CF300-CU

Acceleration Carbon Cellular Copper Electron Electron microscopy Transmission electron microscopy

Corresponding Organization : University of Calgary

Other organizations : University of Bologna

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Variable analysis

independent variables

TeO3^2- exposure

dependent variables

Morphology of Te-nanostructures
Length of TeNRs

control variables

Preparation of cellular suspensions or Te-nanostructure extracts
Mounting of samples on carbon-coated copper grids
Air-drying of samples prior to imaging

controls

Positive control: BCP1 resting cells exposed to TeO3^2-
Negative control: Not explicitly mentioned

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