Culturing AR4-2J Cell Line and E. coli Media

AR4-2J was bought from ATCC (Rockville, MD, USA) and cultured in DMEM/F12 supplemented with 20% fetal bovine serum in a CO₂ incubator under humidified atmosphere (5% CO₂/95% air) at 37 °C, as reported before [1 (link),2 (link),28 (link),33 (link),52 (link)].
Solid E. coli medium LB/Kana and LB/Amp were sterilized and culture plates made. Liquid E. coli medium LB/Kana and LB/Amp had the same composition, but without agar.

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Li Y, & Cui Z.J. (2020). NanoLuc Bioluminescence-Driven Photodynamic Activation of Cholecystokinin 1 Receptor with Genetically-Encoded Protein Photosensitizer MiniSOG. International Journal of Molecular Sciences, 21(11), 3763.

Publication 2020

AR4-2J

Agar Atmosphere E coli Fetal bovine serum

Corresponding Organization : Beijing Normal University

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Variable analysis

independent variables

Culture medium composition (DMEM/F12 supplemented with 20% fetal bovine serum)
Incubation conditions (5% CO2/95% air, 37 °C, humidified atmosphere)

dependent variables

Growth and proliferation of the AR4-2J cell line

control variables

Cell line used (AR4-2J)
Source of cell line (ATCC, Rockville, MD, USA)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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