Quantifying Cell Apoptosis in Leukemia

Cell apoptosis was analyzed as previously described [33 (link)]. Briefly, NB4, MV4-11, Kasumi, and THP-1 cells were treated with dBET1 at indicated concentrations. After 24 h incubation, cells were harvested and washed with cold 1 × PBS buffer. Cells were then suspended in the 1× binding buffer and stained with FITC-Annexin V antibody and PI solution according to the manual of the FITC-Annexin V apoptosis kit (cat. 556420; BD Biosciences, Franklin Lakes, NJ, United States). Cell apoptosis was analyzed by Beckman Gallios™ Flow Cytometer (Beckman, Krefeld, Germany).

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Zhang K., Gao L., Wang J., Chu X., Zhang Z., Zhang Y., Fang F., Tao Y., Li X., Tian Y., Li Z., Sang X., Ma L., Lu L., Chen Y., Yu J., Zhuo R., Wu S., Pan J, & Hu S. (2022). A Novel BRD Family PROTAC Inhibitor dBET1 Exerts Great Anti-Cancer Effects by Targeting c-MYC in Acute Myeloid Leukemia Cells. Pathology and Oncology Research, 28, 1610447.

Publication 2022

Beckman Gallios™ Flow Cytometer

Annexin v fitc Antibody Apoptosis Buffer Cell Cold Thp 1 cells

Corresponding Organization :

Other organizations : Soochow University, Capital Institute of Pediatrics

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Variable analysis

independent variables

DBET1 at indicated concentrations

dependent variables

Cell apoptosis

control variables

NB4, MV4-11, Kasumi, and THP-1 cells
24 h incubation
Cold 1 × PBS buffer
1× binding buffer
FITC-Annexin V antibody
PI solution
FITC-Annexin V apoptosis kit (cat. 556420; BD Biosciences, Franklin Lakes, NJ, United States)
Beckman Gallios™ Flow Cytometer (Beckman, Krefeld, Germany)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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