Serum Biomarker Analyses in Reperfusion Injury

At 120 min after reperfusion, abdominal aorta blood samples were obtained and centrifuged at 4°C at 1800 g for 10 min to harvest the serum [21 (link)]. Levels of lactate dehydrogenase (LDH), creatinine kinase-MB (CK-MB), and cardiac troponin I (cTnI) were measured following the manufacturers’ instructions of the LDH kits (A020-2-2, NanJing JianCheng Bioengineering Institute, Nanjing, Jiangsu, China), CK-MB kits (JianCheng) and cTnI enzyme-linked immunosorbent assay (ELISA) kits (KE1457, Immunoway, Plano, TX, USA), respectively. Optical density (OD) values were measured using a microplate reader, and a standard curve was plotted to calculate the content and activity of each indicator of the samples.

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Song S., Wang Y., Wang H.Y, & Guo L.L. (2022). Role of sevoflurane in myocardial ischemia-reperfusion injury via the ubiquitin-specific protease 22/lysine-specific demethylase 3A axis. Bioengineered, 13(5), 13366-13383.

Publication 2022

LDH kits CK-MB kits

Abdominal aorta Blood Cardiac Creatinine Enzyme linked immunosorbent assay Kinase Lactate dehydrogenase Reperfusion Serum Troponin i Values

Corresponding Organization : Qingdao University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Levels of lactate dehydrogenase (LDH)
Levels of creatinine kinase-MB (CK-MB)
Levels of cardiac troponin I (cTnI)

control variables

Centrifugation at 4°C at 1800 g for 10 min to harvest the serum

controls

No positive or negative controls were explicitly mentioned in the provided information.

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