We amplified 7 neutral microsatellite markers (TA1, PolyA, PfPK2, TA109, 2490, 313, and 383) from each sample (23 (link)) and assayed for size by using an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). We scored alleles manually by using Peak Scanner Software version 1.0 (Applied Biosystems), using a height of 300 relative fluorescence units as the minimal peak threshold.
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