Western blot analysis was carried out as previously interpreted [23 (link), 24 (link)]. Primary antibodies in this experiment comprised of: anti-Sirt6 (Sigma), anti-phosphor-PI3 Kinase p110α, anti-phospho-AKT(Ser473), anti-total pan-AKT, anti phospho-mTOR, and anti-total pan-mTOR, anti-PTEN, anti-phospho-4EBP1, anti-FoxO1, anti-HIF-1α, anti-PARP [specific to the full-length (116 kDa) and the cleaved form (89 kDa) of PARP], anti-p27, anti-CDK2, anti-phospho-ATM, anti-phospho-ATR, anti-phospho-Chk1 (Ser345), anti-phospho-Chk2 (Thr68) and anti-β-tubulin (Cell Signaling Technology, MA, USA), β-actin (Zhongshan Goldenbridge, Beijing, China). Details are shown in Supplementary methods .
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