The LM3 cells were a gift received from Prof. Zheng Shusen [24 (link)], and the SK-Hep1 was obtained from ATCC (Manassas, VA). Both cell lines were maintained in DMEM cell culture medium (Invitrogen, Grand Island, NY) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin and 1% glutamine. All cell lines were cultured in a 5% (v/v) CO2 humidified incubator at 37oC.
Protocol detail
Culturing LM3 and SK-Hep1 Cell Lines
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Corresponding Organization :
Other organizations : Sheng Jing Hospital, Zhejiang University
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Variable analysis
independent variables
- None explicitly mentioned
- None explicitly mentioned
- Cell culture medium (DMEM)
- Fetal bovine serum (10%)
- Penicillin/streptomycin (1%)
- Glutamine (1%)
- Incubator conditions (5% CO2, 37°C, humidified)
- Positive control: None mentioned
- Negative control: None mentioned
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