Annexin V-FITC Apoptosis Assay

Apoptosis of A549 and H1299 cells was examined by the Annexin V-fluorescein isothiocyanate-propidium iodide apoptosis kit (BD Biosciences, NJ, USA). Cells were collected after transfection and washed with cold PBS. The cells were then suspended with 1 × binding buffer (500 μl), mixed with 5 μl Annexin, and preserved at room temperature for 15 min away from light with V‐FITC and 5 μl PI. The percentage of apoptotic cells was assessed on an FACS Calibur flow cytometer [25 (link)].

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Li S., Qiu C., Sun D., Yang S, & Wang L. (2024). circNINL facilitates aerobic glycolysis, proliferation, invasion, and migration in lung cancer by sponging miR-3918 to mediate FGFR1 expression. European Journal of Medical Research, 29, 67.

Publication 2024

Annexin V-fluorescein isothiocyanate-propidium iodide apoptosis kit

Corresponding Organization :

Other organizations : Hainan General Hospital, Hainan Medical University

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Variable analysis

independent variables

Transfection

dependent variables

Percentage of apoptotic cells

control variables

Cells collected after transfection
Cells washed with cold PBS
Cells suspended with 1 × binding buffer (500 μl)
Cells mixed with 5 μl Annexin V‐FITC and 5 μl PI
Cells preserved at room temperature for 15 min away from light

controls

No positive or negative controls were explicitly mentioned.

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