Immunofluorescence Staining of Muscle Markers

The following antibodies were used as described in detail elsewhere^{30 (link),48 (link),52 (link),68 (link),81} : Mouse anti-MyoD (1:250, BD Biosciences #554130), rabbit anti-Myogenin (1:250, Abcam, ab124800), rat anti-Laminin (1:1000, Sigma-Aldrich, L0663), mouse anti-Pax7 (1:100, Developmental Studies Hybridoma Bank), BA-D5 (MyHC-I, mouse IgG2b, 1:40, DSHB), SC-71 (MyHC-IIA, mouse IgG1, 1:40, DSHB), BF-F3 (MyHC-IIB, mouse IgM, 1:40, DSHB), AlexaFluor 488-conjugated goat anti-mouse IgG (1:1500, Life Technologies, A-11001), AlexaFluor 488-conjugated goat anti-rabbit IgG (1:1500, Life Technologies, A-11034), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, Life Technologies, A-21247), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, Thermo Fisher Scientific, A-21141), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher Scientific, A-21042), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher Scientific, A-21125).