After paraffin removal and rehydration, sections were either stained with hematoxylin and eosin for histological analysis or treated with 0.3% H2O2 for immunohistochemistry. Slides were processed as described [22 (link)] for antigen retrieval, permeabilization, blocking, and antibody staining (listed in Supplementary Table S1). For both histology and immunohistochemistry, slides were mounted in Dako aqueous Medium (Agilent Technologies, Santa Clara, CA, USA) and scanned with the panoramic P250 digital slide scanner (3DHistech, Budapest, Hungary).
Other organizations :
University of Turku, Walloon Excellence in Lifesciences and Biotechnology, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University, Memorial Sloan Kettering Cancer Center, Cliniques Universitaires Saint-Luc
Staining method: hematoxylin and eosin or H2O2 treatment for immunohistochemistry
dependent variables
Histological analysis
Immunohistochemistry
control variables
Paraffin removal and rehydration
Antigen retrieval, permeabilization, blocking, and antibody staining (as described in the referenced publication)
Mounting slides in Dako aqueous Medium
Scanning slides with the panoramic P250 digital slide scanner
controls
Positive control: Not specified
Negative control: Not specified
Annotations
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