Chemical Synthesis and Biochemical Analysis

Ipomoeassin F was chemically synthesized as described previously12 (link), 13 (link). PDI inhibitor CCF 642 (346640-08-2) was purchased from Tocris Bioscience (Shanghai, China). Thioflavin T (Th T) and Cycloheximide (CHX) were obtained from Sigma-Aldrich (USA). mRFP-GFP-MAP1LC3B plasmid was used as previously described14 (link). All antibody information is indicated as following: PDIA6 (#18233-1-AP, Proteintech), Calnexin (#10427-2-AP, Proteintech), GRP78/Bip (#11587-1-AP, Proteintech), ERp72 (PDIA4) (#2798, CST), eIF2α (#5324, CST), pho-eIF2α (#3597, CST), cleaved-caspase3 (#9664, CST), LC3B (#2775, CST), GAPDH (sc-3650620, Santa Cruz), α-Tubulin (sc-5286, Santa Cruz), anti-mouse linked with HRP (sc-2005, Santa Cruz), anti-rabbit IgG linked with HRP (sc-2004, Santa Cruz), Alexa Fluor 488 donkey anti-mouse antibody (#A21202, Thermo Fisher), and Alexa Fluor 594 donkey anti-rabbit antibody (#A21207, Thermo Fisher). Details for the use of antibodies can be found in Supplementary Table S2.

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Tao S., Yang E.J., Zong G., Mou P.K., Ren G., Pu Y., Chen L., Kwon H.J., Zhou J., Hu Z., Khosravi A., Zhang Q., Du Y., Shi W.Q, & Shim J.S. (2023). ER translocon inhibitor ipomoeassin F inhibits triple-negative breast cancer growth via blocking ER molecular chaperones. International Journal of Biological Sciences, 19(13), 4020-4035.

Publication 2023

Thioflavin T (Th T) Cycloheximide (CHX) PDIA6 Calnexin GRP78/Bip GAPDH α-Tubulin Alexa Fluor 488 donkey anti-mouse antibody Alexa Fluor 594 donkey anti-rabbit antibody

Alexa 594 Alexa fluor 488 Anti antibody Anti igg Antibodies Antibody Calnexin Caspase3 Cycloheximide Donkey Erp72 Gapdh Grp78 Ipomoeassin f Mouse Plasmid Rabbit Thioflavin t α tubulin

Corresponding Organization :

Other organizations : University of Macau, University of Maryland, College Park, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Yonsei University, University of Arkansas at Fayetteville, Feinstein Institute for Medical Research, Northwell Health, Ball State University

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Variable analysis

independent variables

Ipomoeassin F (chemically synthesized)
PDI inhibitor CCF 642 (346640-08-2)
Thioflavin T (Th T)
Cycloheximide (CHX)
MRFP-GFP-MAP1LC3B plasmid

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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