Kinetic Evaluation of MAO Inhibitors

The potential of the test compounds for human MAO inhibition was evaluated via a chemiluminescence technique using the MAO-Glo kit (Promega, Madison, WI, USA). Detailed experimental conditions and procedures were reported previously [64 (link),65 (link)]. The test compounds were evaluated at a concentration of 6, 30, and 120 µM. Selegiline was used as a positive control. The kinetic analysis of hMAO inhibition was analyzed at different concentrations of hMAO substrate depending on the isozyme (40, 80, and 160 µM for hMAO-A and 4, 8, and 16 µM for hMAO-B) following the same method of enzyme inhibition. The concentrations of the test compounds for the kinetic study are presented in Figure 2 and Figure 3. Kinetic parameters were analyzed using SigmaPlot (v12.0, SPP Inc., Chicago, IL, USA).