Super-resolution Imaging of Chromatin Spreads

After NIH2/4 cells were fixed and quenched, cells were treated with
Trypsin (GIBCO) and re-suspended with culture medium. Chromatin spreads were
prepared according to the methods previously described33 (link). Super-resolution imaging was performed on a Zeiss Elyra
P.1 microscope equipped with an oil-immersion objective, and images were
analysed according to the procedures previously described33 (link).

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Michelini F., Pitchiaya S., Vitelli V., Sharma S., Gioia U., Pessina F., Cabrini M., Wang Y., Capozzo I., Iannelli F., Matti V., Francia S., Shivashankar G.V., Walter N.G, & d’Adda di Fagagna F. (2017). Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. Nature cell biology, 19(12), 1400-1411.

Publication 2017

ElyraP.1 microscope

Cells Chromatin Culture medium Immersion Microscope

Corresponding Organization :

Other organizations : FIRC Institute of Molecular Oncology, University of Michigan–Ann Arbor, Istituto di Genetica Molecolare, National University of Singapore

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Variable analysis

independent variables

Treatment with Trypsin (GIBCO)

dependent variables

Chromatin spreads
Super-resolution imaging

control variables

Cells were fixed and quenched prior to treatment

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