The procedure was adapted from a previous report (49 (link)). Briefly, RNA species smaller than 200 nt were extracted from the total RNA using RNA Clean & Concentrator Kits (Zymo Research). The tRNA fraction was further extracted from the small RNAs by using 6% TBE–urea gel and extracting the migrated tRNAs from the gel using a ZR small-RNA PAGE Recovery Kit (Zymo Research).
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