Plasmid Transfection and Selection

mApple-Dectin1A-C-10 was a gift from Michael Davidson (Addgene, Watertown, MA, USA; plasmid # 54883). pEGFP-DC-SIGN was a generous gift from Ken Jacobson [12 (link)]. pUNO1-hDectin-1a was purchased from Invivogen (San Diego, CA, USA; #puno1-hdectin1a). mCardinal-Lifeact-7 was a gift from Michael Davidson (Addgene, Watertown, MA, USA; plasmid #54663). Transfection with plasmid was performed following standard protocols for FuGENE 6 (Promega, Madison, WI, USA; #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate) (Thermo Fisher Scientific, Waltham, MA, USA; #10131035; for pEGFP-DC-SIGN) at 400 μg/mL or Blasticidin (Invivogen, San Diego, CA, USA; #ant-bl-05; for pUNO1-hDectin-1a) at 20 μg/mL for 2 weeks.