Demethylation Induction in Breast and Leukemia Cell Lines

BC cell line MCF-7,ZR-75-1, MDA-MB-231 and myeloid leukemia cell line K562 were all obtained from the Experimental Center, Shunde Hospital of Guangzhou University of Chinese Medicine (Foshan, China). Three breast cell lines were maintained in DMEM medium with 10% fetal bovine serum (FBS). Cells were seeded in 6-well plates and, 12 h after plating, demethylation was induced with 10 μM 5-aza for 24 h. Stable expression of TSPAN7 was confirmed by RT-qPCR and Western blot.

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Ren C., Gao A., Fu C., Teng X., Wang J., Lu S., Gao J., Huang J., Liu D, & Xu J. (2023). The biomarkers related to immune infiltration to predict distant metastasis in breast cancer patients. Frontiers in Genetics, 14, 1105689.

Publication 2023

Breast Cell lines Cells Chinese Demethylation Fetal bovine serum Medicine Myeloid leukemia Western blot

Corresponding Organization : Guangzhou University of Chinese Medicine

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Variable analysis

independent variables

Treatment with 10 μM 5-aza for 24 h

dependent variables

Expression of TSPAN7 (measured by RT-qPCR and Western blot)

control variables

Cell lines (MCF-7, ZR-75-1, MDA-MB-231, and K562)
Cell culture conditions (DMEM medium with 10% FBS)
Cell seeding (in 6-well plates)

controls

Positive control: Not specified
Negative control: Not specified

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