During the patch clamp experiments the cells were imaged on an IX71 microscope with a 60× 1.35-numerical aperture (NA) oil-immersion lens (Olympus). Fluorescence excitation was delivered using a 75 W Xenon Arc lamp (Cairn). The filter set used was that reported for ArcLight [7 (link)]. The excitation filter was FF02-472/30 (Semrock). The emission filter was FF01-496/LP (Semrock). The dichroic was FF495-Di03 (Semrock). The objective C-mount image was de-magnified by an Optem zoom system A45699 (Qioptiq LINOS, Inc., Fairport, NY) and projected onto the e2v CCD39 chip of NeuroCCD-SM 80 pixel × 80 pixel camera (RedShirtImaging,). The imaging apparatus was mounted on a Vibraplane Bench Top vibration isolation platform (Minus K Technology). The mechanical shutter in the incident light patch was mounted on a separate table. Images were recorded at a frame rate of 1kfps. The excitation light was 1 mW/mm2.
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High-Speed Fluorescence Imaging of ArcLight
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Other organizations : Korea Institute of Science and Technology, Korea University, Korea University of Science and Technology
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Variable analysis
independent variables
- None explicitly mentioned
- Fluorescence of ArcLight
- Cells imaged on an IX71 microscope with a 60× 1.35-numerical aperture (NA) oil-immersion lens (Olympus)
- Fluorescence excitation delivered using a 75 W Xenon Arc lamp (Cairn)
- Filter set used was that reported for ArcLight [7]
- Excitation filter: FF02-472/30 (Semrock)
- Emission filter: FF01-496/LP (Semrock)
- Dichroic: FF495-Di03 (Semrock)
- Objective C-mount image de-magnified by an Optem zoom system A45699 (Qioptiq LINOS, Inc., Fairport, NY) and projected onto the e2v CCD39 chip of NeuroCCD-SM 80 pixel × 80 pixel camera (RedShirtImaging,)
- Imaging apparatus mounted on a Vibraplane Bench Top vibration isolation platform (Minus K Technology)
- Mechanical shutter in the incident light patch mounted on a separate table
- Images recorded at a frame rate of 1kfps
- Excitation light intensity: 1 mW/mm^2
- None explicitly mentioned
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