Ultrastructural Analysis of C. albicans Treated with X33 AMOP

C. albicans cells were incubated with X33 AMOP (MIC) at 37°C for 0, and 4 h (Ma et al., 2020 (link)). Thereafter, the cells were fixed with 2.5% glutaraldehyde and dehydrated with 50%, 60%, 70%, 80%, 90%, 95% and 100% ethanol gradients, followed by drying using 98% hexamethyldisilazane (HMDS). The samples were then coated with gold and imaged with the TESCAN vega 3 LMU scanning electron microscope (SEM).
Since the changes in internal cellular morphology can be used to evaluate the antifungal effect of X33 AMOP on C. albicans, the cells were incubated with X33 AMOP (MIC) in the YPD medium at 37°C for 0, 4, and 12 h (Su et al., 2020 (link)). The cells were then fixed with 2.5% glutaraldehyde and 1% osmic acid solution at 4°C. After dehydrating with 50%, 60%, 70%, 80%, 90%, 95% and 100% ethanol, the samples were treated with an embedding agent and acetone. The samples were then sliced in an ultra-thin microtome and stained with lead citrate solution, followed by a saturated solution of uranyl acetate and 50% for 10 min each. Thereafter, the stained samples were observed by transmission electron microscope (TEM).