All participants completed a uniform questionnaire detailing history of diabetes, medication use, smoking status, and alcohol consumption. Body height and weight were measured without shoes and outer clothing. BMI was calculated according to weight in kilograms divided by square of height in meters. Waist circumference was measured using a soft tape at midway between the lowest rib and iliac crest in standing position. All blood samples were obtained after at least 12 h of fasting. Serum total cholesterol, HDL-cholesterol, triglyceride(TG) were measured using oxidase method32 (link). LDL cholesterol was calculated using the Friedewald equation. Liver enzymes (ALT, AST) were measured by Ultraviolet (UV) lactate and malate dehydrogenase methods on a model 7600 automated bio-analyser (Hitachi, Tokyo, Japan). Plasma glucose concentrations were measured using glucose oxidase method. Serum insulin concentrations were determined using an electrochemiluminescence immunoassay33 (link).
Free full text: Click here
