COVID-19 Convalescent Plasma IgG Isolation

Plasma samples collected from COVID-19 convalescent and healthy donors are described in (18 (link)). Human IgGs were isolated from heat-inactivated plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) as described (24 ).

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Cohen A.A., Gnanapragasam P.N., Lee Y.E., Hoffman P.R., Ou S., Kakutani L.M., Keeffe J.R., Wu H.J., Howarth M., West A.P., Barnes C.O., Nussenzweig M.C, & Bjorkman P.J. (2021). Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice. bioRxiv.

Publication Preprint 2021

HiTrap MabSelect SuRe columns

Covid 19 Donors Heat plasma Human Plasma

Corresponding Organization : California Institute of Technology

Other organizations : University of Oxford, Rockefeller University

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Variable analysis

independent variables

Plasma samples collected from COVID-19 convalescent donors
Plasma samples collected from healthy donors

dependent variables

Human IgGs isolated from heat-inactivated plasma samples

control variables

5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences) used to isolate human IgGs

controls

No positive or negative controls were explicitly mentioned in the given information.

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