All Raman spectra were obtained from 10 points randomly distributed over the polished surface per bone sample using a confocal Raman system (Renishaw InVia Raman microscope) with a holographic grating (1200 lines/mm) providing ~1 cm−1 spectral resolution and a 830 nm laser source (Renishaw). Each spectrum was obtained as the average of 10 consecutive spectra with each collected for 5 seconds using a 20X objective (NA=0.40), focusing the laser into a ~2.5 µm spot on each sample. Daily silicon and laser power measurements (~35 mW) before and after data collection ensured wavenumber calibration and light throughput, respectively. Background fluorescence was removed from all spectra using fourth order polynomial fitting algorithm (53 (link)).
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Raman Spectroscopy of Bone Samples
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Corresponding Organization : Vanderbilt University
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Variable analysis
independent variables
- Bone sample
- Raman spectra
- Confocal Raman system (Renishaw InVia Raman microscope) with a holographic grating (1200 lines/mm) providing ~1 cm^-1 spectral resolution and a 830 nm laser source (Renishaw)
- Each spectrum was obtained as the average of 10 consecutive spectra with each collected for 5 seconds using a 20X objective (NA=0.40), focusing the laser into a ~2.5 µm spot on each sample
- Daily silicon and laser power measurements (~35 mW) before and after data collection ensured wavenumber calibration and light throughput, respectively
- Background fluorescence was removed from all spectra using fourth order polynomial fitting algorithm
- Daily silicon and laser power measurements before and after data collection to ensure wavenumber calibration and light throughput
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