Prioritizing Genes for Mitochondrial Calcium Uptake
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Corresponding Organization :
Other organizations : Center for Human Genetics, Massachusetts General Hospital, Broad Institute, University of Colorado Boulder
Protocol cited in 9 other protocols
Variable analysis
- RNAi knockdown of 13 human genes
- Rescue of MICU1 RNAi Ca2+ phenotype with custom synthesized cDNA
- Agonist-induced rises in mitochondrial Ca2+ measured by luminescence
- Ca2+ uptake in permeabilized HeLa cells measured using Calcium Green 5N
- FRET-based measurements of mitochondrial, cytosolic, and ER Ca2+ in single HeLa cells
- Mitochondrial respiration, morphology, and mtDNA copy number
- Mitochondrial membrane potential measured by flow cytometry
- Single cell measurements of NADH
- Mitochondrial aequorin (mt-AEQ) expression in HeLa cell line
- Lentiviral constructs from Broad Institute's RNAi Consortium
- Rescue of MICU1 RNAi Ca2+ phenotype with custom synthesized cDNA
- Not explicitly mentioned
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