Human patient samples were collected at the Department of Neurosurgery of the University of Pennsylvania. The collection of human tissues, in compliance with the tissue banking protocol, was approved by the University of Pennsylvania Institutional Review Board, and written informed consent was obtained from each participant13 (link),14 (link). ECs were isolated and verified as previously described13 (link),14 (link). In brief, tumor-derived single-cell suspensions were prepared by the tissue bank. Cell suspensions were subjected to magnetic-activated cell sorting using anti-CD31 antibody-conjugated magnetic beads (1:100; Miltenyi Biotech; 130-091-935). Sorted ECs were verified by Dil-Ac-LDL (Alfa Aesar; J65597) absorption and von Willebrand factor staining.
Protocol detail
Isolation and Verification of Endothelial Cells from Tumor Samples
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Anti antibody
Cell
Human
Institutional review board
Neurosurgery
Patient
Tumor
Von willebrand factor
Corresponding Organization :
Other organizations : University of Pennsylvania
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Variable analysis
independent variables
- Tumor-derived single-cell suspensions
- Endothelial cells (ECs) isolated and verified
- Tissue banking protocol approved by Institutional Review Board
- Written informed consent obtained from each participant
- Positive control: Dil-Ac-LDL absorption by sorted ECs
- Positive control: von Willebrand factor staining of sorted ECs
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