Extraction and Quantitation of Analytes

A pair of different methods were used for the extraction of serum, urine, and feces. Samples used for the quantitation of targeted analytes were extracted using a modified version of the liquid extraction developed by D'eon and Mabury (2011)^{28 (link)} based on protein precipitation with cold acetonitrile. Serum and urine samples used for untargeted analysis required a concentration step to increase the probabilities of detecting unknown metabolites. Samples were extracted with a solid phase-extraction method using an OASIS HLB (Waters) cartridge. All details on extraction are available in the Supporting Information. Limits of quantitation and recoveries for rat serum, urine, and feces are available on Table S.3.

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Dagnino S., Strynar M.J., McMahen R.L., Lau C.S., Ball C., Garantziotis S., Webster T.F., McClean M.D, & Lindstrom A.B. (2016). Identification of Biomarkers of Exposure to FTOHs and PAPs in Humans Using a Targeted and Nontargeted Analysis Approach. Environmental science & technology, 50(18), 10216-10225.

Publication 2016

OASIS HLB

Acetonitrile Cold Feces Oasis Protein Serum Solid phase extraction Urine

Corresponding Organization :

Other organizations : Oak Ridge Associated Universities, Agilent Technologies (United States), National Institute of Environmental Health Sciences, Boston University

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Variable analysis

independent variables

Extraction method used for serum, urine, and feces samples

dependent variables

Quantitation of targeted analytes
Detection of unknown metabolites

control variables

None explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

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