Whole-Mount In Situ Hybridization Analysis of Embryonic and Ovary Tissues

Embryonic and ovary WMISH were performed as described previously (38 (link), 53 (link), 54 (link)). Phospho-p44/42 MAPK Rabbit mAb (Cell Signaling Technology, Inc.) with 1:1,000 dilution was used to analyze the Torso signaling during the early embryogenesis. Images were acquired with a Zeiss Axioplan 2 LSM510.

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Ansari S., Troelenberg N., Dao V.A., Richter T., Bucher G, & Klingler M. (2018). Double abdomen in a short-germ insect: Zygotic control of axis formation revealed in the beetle Tribolium castaneum. Proceedings of the National Academy of Sciences of the United States of America, 115(8), 1819-1824.

Publication 2018

Phospho-p44/42 MAPK Rabbit mAb Axioplan 2 LSM510

Dilution Embryogenesis Embryonic Ovary P44 mapk Rabbit Torso

Corresponding Organization :

Other organizations : University of Göttingen, Friedrich-Alexander-Universität Erlangen-Nürnberg

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Variable analysis

independent variables

Embryonic and ovary whole-mount in situ hybridization (WMISH) protocols

dependent variables

Torso signaling during early embryogenesis

control variables

Phospho-p44/42 MAPK Rabbit mAb (Cell Signaling Technology, Inc.) with 1:1,000 dilution
Zeiss Axioplan 2 LSM510 for image acquisition

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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