The liquid chromatography-mass spectrometry (LC-MS) grade solvents (i.e., isopropanol, methanol, water, and acetonitrile) and additives (i.e., ammonium acetate, acetic acid, and formic acid) used in this work were purchased from Sigma Aldrich (Poznan, Poland). The probes were prepared using N, N-dimethylformamide ACS reagent and polyacrylonitrile. In addition, the phosphate-buffered saline used in the experiments was acquired from Sigma Aldrich (Poznan, Poland).
The standards selected for this work included Sphingosine (d17:1), LPE (17:1), PC (C16–18:1), PG (17:0–20:4), L-carnitine(trimethyl-d9), L-carnitine inner salt, phenylalanine, and phenylalanine(d8). The lipid short-hand notation presented herein was used in this manuscript.
Coated blade spray-mass spectrometry probes (CBS-MS) were prepared according to a procedure described elsewhere [18 (link)]. Two types of sorbents were used in the experiments: octadecyl (C18), which was purchased from Anchem (Toruń, Poland), and hydrophilic–lipophilic balance particles (HLB), which were manufactured and kindly provided by the University of Waterloo [32 (link)].
The standards selected for this work included Sphingosine (d17:1), LPE (17:1), PC (C16–18:1), PG (17:0–20:4), L-carnitine(trimethyl-d9), L-carnitine inner salt, phenylalanine, and phenylalanine(d8). The lipid short-hand notation presented herein was used in this manuscript.
Coated blade spray-mass spectrometry probes (CBS-MS) were prepared according to a procedure described elsewhere [18 (link)]. Two types of sorbents were used in the experiments: octadecyl (C18), which was purchased from Anchem (Toruń, Poland), and hydrophilic–lipophilic balance particles (HLB), which were manufactured and kindly provided by the University of Waterloo [32 (link)].
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