Generating Drosophila Genetic Reporters

WT flies used were Canton S (CS). Lar and sns mutants have been previously described: Lar^13.2, Lar⁴⁵¹, and sns^xb3 (gift of Dr. Susan Abmayr). The following lines were obtained from the Bloomington Stock Center: sns^Df, C155-GAL4, tubulin-GAL4, OK107-GAL4, sns^{MiMIC MI03001}, Lar^MiMIC02154, sns^EY08142, UAS-Lar RNAi (TRiP.HMS02186), UAS-Lar RNAi (TRiP.HMS00822), and UAS-Kirre RNAi (TRiP.HMC05791). Sns RNAi lines were from the Vienna Drosophila Resource Center: UAS-Sns RNAi (KK109442) and UAS-Sns RNAi (GD877). T2A-GAL4 lines were generated as described in Diao et al., 2015 (link). Briefly, flies carrying the MiMIC insertion were crossed with flies bearing the triplet ‘Trojan exon’ donor. The F₁ males from this cross carrying both genetic components were crossed to females carrying germline transgenic sources of Cre and ϕC31. The F₂ males from this cross that had all four genetic components were then crossed to a UAS-2xEGFP reporter line, and the resulting progeny were screened for T2A-GAL4 transformants.