To visualize nuclei, the epidermis was stained with DAPI (5 µg/mL, sigma, USA). Materials were soaked in the dye liquid phosphate buffer solution (PBS) (pH 7.0; DAPI: PBS (v/v) = 1∶1000) and kept in darkness for 20 min. Pieces of onion epidermis were arranged on slides to make wet mounts, the made slides were observed and photographed in dark-field of fluorescence microscope (Olympus BX 61, Japan).
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