Climbing Assay for Fly Lifespan

Climbing assays were carried out according to previously described methods (Ng et al. 2009 (link)). In general, 30 flies per group were used for the assay and the experiment was replicated with three different sets of flies. To study the effects of drugs, flies were fed with cornmeal-agar medium supplemented with 250 μM C2 Ceramide (N-acetyl-d-sphingosine, Sigma-Aldrich), 250 μM Fingolimod (FTY720), HCl (Selleckchem) or 250 μM S6K1 Inhibitor (PF-4708671, Tocris) at day 25 post-eclosion till day 50.

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Sim J.P., Ziyin W., Basil A.H., Lin S., Chen Z., Zhang C., Zeng L., Cai Y, & Lim K.L. (2019). Identification of PP2A and S6 Kinase as Modifiers of Leucine-Rich Repeat Kinase-Induced Neurotoxicity. Neuromolecular Medicine, 22(2), 218-226.

Publication 2019

C2 Ceramide Fingolimod (FTY720), HCl S6K1 Inhibitor (PF-4708671,

Agar Assay C2 ceramide Fingolimod Flies Fty720 Pf 4708671 Sphingosine

Corresponding Organization :

Other organizations : National Neuroscience Institute, Nanjing Tech University, Temasek Life Sciences Laboratory

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Variable analysis

independent variables

250 μM C2 Ceramide (N-acetyl-d-sphingosine, Sigma-Aldrich)
250 μM Fingolimod (FTY720), HCl (Selleckchem)
250 μM S6K1 Inhibitor (PF-4708671, Tocris)

dependent variables

Climbing assay

control variables

Cornmeal-agar medium
30 flies per group
Three different sets of flies

controls

No positive or negative controls were explicitly mentioned in the provided information.

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