Peripheral blood (10 mL) was drawn from the subjects using standardized phlebotomy procedures in BD K2EDTA Tubes (BD, Franklin Lakes, NJ, USA), as described in our previous studies [20 (link),28 (link),36 (link)]. The blood specimens were processed within 2 h of collection by centrifugation at 1300× g at for 10 min 4 °C. Plasma was transferred to a fresh tube and stored at −80 °C until use.
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