Ceramide Profiling by UPLC-ESI-QTOF-MS

Ceremide profiles were obtained by using the same Agilent UPLC-ESI-QTOF-MS system as in the case of phospholipid profiling (Agilent 1290; Agilent 6540; Agilent Technologies, Santa Clara, CA, USA). Ceremides were separated by RPLC on the RP C18 column (Acquity BEH Shield 2.1 × 100 mm; 1.7 μm; Waters, Milford, MA, USA) using methanol and water with 20 mM ammonium formate pH 5. The QTOF operating parameters and identification of ceramide species was previously described in detail^{29 (link)}.

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Łuczaj W., Dobrzyńska I, & Skrzydlewska E. (2023). Differences in the phospholipid profile of melanocytes and melanoma cells irradiated with UVA and treated with cannabigerol and cannabidiol. Scientific Reports, 13, 16121.

Publication 2023

UPLC-ESI-QTOF-MS system Agilent 1290 Agilent 6540

Ammonium formate Ceramide Methanol Phospholipid

Corresponding Organization :

Other organizations : Medical University of Białystok, University of Białystok

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Variable analysis

independent variables

Ceremide profiles

dependent variables

Ceremide species

control variables

UPLC-ESI-QTOF-MS system
RP C18 column (Acquity BEH Shield 2.1 × 100 mm; 1.7 μm; Waters, Milford, MA, USA)
Methanol and water with 20 mM ammonium formate pH 5
QTOF operating parameters

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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