Histological Evaluation of NAFLD

Paraffin-embedded sections of the liver and white adipose tissue (4 μm) were processed by haematoxylin and eosin for histological evaluation, as described previously^{41 (link),42 (link)}. Diagnostic classification of NAFLD was performed by applying a semiquantitative scoring system that grouped histological features into broad categories (steatosis, hepatocellular injury, portal inflammation, fibrosis and miscellaneous features)^{43 (link)}. The white adipose tissue sections were observed with an optical microscope (Microscope Axioscope 5/7 KMAT, Carl Zeiss, Milan, Italy) connected to a digital camera (Microscopy Camera Axiocam 208 color, Carl Zeiss, Milan, Italy) for evaluation.

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Chiodi V., Rappa F., Lo Re O., Chaldakov G.N., Lelouvier B., Micale V., Domenici M.R, & Vinciguerra M. (2023). Deficiency of histone variant macroH2A1.1 is associated with sexually dimorphic obesity in mice. Scientific Reports, 13, 19123.

Publication 2023

Microscope Axioscope 5/7 KMAT Microscopy Camera Axiocam 208 color

Camera Diagnostic Embedded paraffin Eosin Fibrosis Haematoxylin Inflammation Injury Liver Microscopy Nafld Optical microscope Steatosis White adipose tissue

Corresponding Organization : Liverpool John Moores University

Other organizations : Istituto Superiore di Sanità, University of Palermo, University of Catania

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Variable analysis

independent variables

Paraffin-embedded sections of the liver and white adipose tissue (4 μm)

dependent variables

Histological evaluation of the liver and white adipose tissue
Diagnostic classification of NAFLD based on a semiquantitative scoring system

control variables

Haematoxylin and eosin staining method for histological evaluation, as described previously in references 41 and 42
Optical microscope (Microscope Axioscope 5/7 KMAT, Carl Zeiss, Milan, Italy) connected to a digital camera (Microscopy Camera Axiocam 208 color, Carl Zeiss, Milan, Italy) for evaluation of the white adipose tissue sections

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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