Profiling BCR-ABL and Associated Signaling

BCR-ABL protein and relevant signal molecules were assessed by western blotting [1 (link)] using rabbit anti-phospho-BCR-ABL (#2868), anti-MEK1/2 (#9122), anti-BCR-ABL (#2862), anti-STAT5(#94205), anti-phospho-STAT5 (#9314), anti-phospho-ERK (#9101), anti-phospho-CRKL (#3181), anti-Caspase-3 (#9662), anti-CRKL (#38710) and anti-PARP (#9532) antibody with 1:1000 dilution (Cell Signaling Technology, USA). Horseradish peroxidase-conjugated α-rabbit antibody (Introvigen, USA) was used as a secondary antibody at 1:5000 dilution. Detection of blot was performed using super ECL plus western blotting substrate (Baoguang, China).

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Luo Z., Gao M., Huang N., Wang X., Yang Z., Yang H., Huang Z, & Feng W. (2019). Efficient disruption of bcr-abl gene by CRISPR RNA-guided FokI nucleases depresses the oncogenesis of chronic myeloid leukemia cells. Journal of Experimental & Clinical Cancer Research : CR, 38, 224.

Publication 2019

anti-phospho-BCR-ABL anti-MEK1/2 anti-BCR-ABL anti-STAT5 anti-phospho-STAT5 anti-phospho-ERK anti-phospho-CRKL anti-Caspase-3 anti-CRKL anti-PARP

Antibody Bcr protein Caspase 3 Crkl Dilution Horseradish peroxidase Mek1 Rabbit Stat5

Corresponding Organization :

Other organizations : Chongqing Medical University, First Affiliated Hospital of Chongqing Medical University, Children's Hospital of Chongqing Medical University

Top 5 similar protocols

Variable analysis

independent variables

Rabbit anti-phospho-BCR-ABL (#2868)
Rabbit anti-MEK1/2 (#9122)
Rabbit anti-BCR-ABL (#2862)
Rabbit anti-STAT5 (#94205)
Rabbit anti-phospho-STAT5 (#9314)
Rabbit anti-phospho-ERK (#9101)
Rabbit anti-phospho-CRKL (#3181)
Rabbit anti-Caspase-3 (#9662)
Rabbit anti-CRKL (#38710)
Rabbit anti-PARP (#9532)

dependent variables

BCR-ABL protein
Relevant signal molecules

control variables

Rabbit anti-bodies at 1:1000 dilution
Horseradish peroxidase-conjugated α-rabbit antibody at 1:5000 dilution
Super ECL plus western blotting substrate (Baoguang, China) for detection

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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