Chloroplast Genome Sequencing of Ulmus mianzhuensis

Fresh leaves of U. mianzhuensis were collected from Central Forest Tree Nursery in Sichuan, China and stored in silica gel prior. The voucher specimen (Deng11468) was deposited in the Herbarium of Henan Agricultural University. For this species, 50 mg dried leaves were ground and high-quality genomic DNAs were extracted using a Plant Genomic DNA Extraction Kit (Tiangen, Beijing, China), and then were subsequently sent to Novogene (http://www.novogene.com, China) for short insert (350 bp) library construction and next-generation sequencing by Illumina Hiseq 4000 genome analyzer platform (Illumina, San Diego, CA) by Novogene, Beijing, China. Raw reads from the paired-end for quality were filtered with the NGSQC ToolKit by removing adapter sequences and low-quality reads with Q value B20 [52 (link)]. Then the clean data were assembled by NOVOPlasty 2.6.3 [53 (link)]and annotated by GeSeq [54 (link)], and the results were manually checked and verified in Geneious v.9.1 according to Ulmus parvifolia (MT165940) [55 (link)]. The visualization of the cp genome map was performed in OGDRAW [56 (link)].