Puromycin (P8833), Baf A1 (J61835), polybrene (H9268), irinotecan (I1406), all-trans-retinoic acid (R2625), and N-acetyl-l -cysteine (A9165) were all purchased from Sigma-Aldrich (USA). Anti-Top1mt–specific antibody (47 (link), 52 (link)) (Top1mt, polyclonal mouse) was a gift from Y. Pommier [Center for Cancer Research, National Cancer Institute, National Institutes of Health (NIH), USA]. Rabbit polyclonal antibodies [TDP1 (Ab4166), PGC1α (Ab54481), NRF1 (Ab34682), and mtTFAM (Ab47517)] and mouse monoclonal antibodies [COX IV (Ab14744), DRP1 (Ab56788), Mfn1 (Ab57602), and double-stranded DNA (dsDNA) (Ab27156)] were purchased from Abcam (Cambridge, USA). Mouse monoclonal antibodies [FLAG (M2) (F3165)] and rabbit polyclonal antibodies [FLAG (F7425) and LC3B (L7543)] were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse monoclonal γH2AX (05-636) antibody was purchased from Millipore, USA. Rabbit monoclonal GFP (G10362) and anti OXPHOS complex kit (457999) antibodies were purchased from Invitrogen. Mouse monoclonal actin (SC-8432) and secondary antibodies [horseradish peroxidase–conjugated anti-rabbit immunoglobulin G (IgG) or anti-mouse IgG] were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). MitoTracker red (M22426), MitoTracker green (M7514), TMRM (T668), CM-H2DCFDA (C6827), and LysoTracker red DND-99 (L7528) were obtained from Molecular Probes.
Protocol detail
Evaluating Mitochondrial Function and Oxidative Stress
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Actin
All trans retinoic acid
Anti immunoglobulin
Antibodies
Antibody
Cancer
Dsdna
H2dcfda
Horseradish peroxidase
Immunoglobulin g
Irinotecan
Lysotracker
Molecular probes
Monoclonal antibodies
Mouse
N acetyl l cysteine
Pgc1α
Polybrene
Puromycin
Rabbit
Red dnd 99
Corresponding Organization : Indian Association for the Cultivation of Science
Other organizations : Indian Institute of Science Education and Research Pune, Indian Institute of Technology Gandhinagar
Top 5 similar protocols
Variable analysis
independent variables
- Puromycin (P8833)
- Baf A1 (J61835)
- Polybrene (H9268)
- Irinotecan (I1406)
- All-trans-retinoic acid (R2625)
- N-acetyl-L-cysteine (A9165)
- Top1mt (polyclonal mouse antibody)
- PGC1α
- MtTFAM
- COX IV
- Double-stranded DNA (dsDNA)
- FLAG (M2)
- FLAG (F7425)
- γH2AX
- OXPHOS complex
- Actin
- Horseradish peroxidase–conjugated anti-rabbit immunoglobulin G (IgG) or anti-mouse IgG
- MitoTracker red (M22426)
- MitoTracker green (M7514)
- TMRM (T668)
- CM-H2DCFDA (C6827)
- LysoTracker red DND-99 (L7528)
- Anti-Top1mt–specific antibody (47, 52) (Top1mt, polyclonal mouse)
- Not specified
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