Mouse Oligodendrocyte Precursor Cell Culture

The FBD-102b cell line (a mouse brain oligodendrocyte precursor cell line) was kindly provided by Dr. Y. Tomo-oka (Tokyo University of Science, Chiba, Japan; Riken, Saitama, Japan). FBD-102b cells on cell culture dishes (Greiner, Oberösterreich, Germany) or glass coverslips (Matsunami Glass, Osaka, Japan) were cultured in DMEM/F-12 (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS (Thermo Fisher Scientific, Waltham, MA, USA) and PenStrep (Thermo Fisher Scientific) in the presence or absence of ibuprofen (300 micromolar, Nacalai Tesque) [25 (link),26 (link)]) in 5% CO₂ at 37 °C.

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Sawaguchi S., Suzuki R., Oizumi H., Ohbuchi K., Mizoguchi K., Yamamoto M., Miyamoto Y, & Yamauchi J. (2022). Hypomyelinating Leukodystrophy 8 (HLD8)-Associated Mutation of POLR3B Leads to Defective Oligodendroglial Morphological Differentiation Whose Effect Is Reversed by Ibuprofen. Neurology International, 14(1), 212-244.

Publication 2022

cell culture dishes glass coverslips DMEM/F-12 FBS PenStrep ibuprofen

Brain Cell line Cells culture Dishes Ibuprofen Mouse Oligodendrocyte precursor

Corresponding Organization : National Center For Child Health and Development

Other organizations : Tsumura Research Institute (Japan)

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Variable analysis

independent variables

Ibuprofen (300 micromolar)

dependent variables

Not explicitly mentioned

control variables

Cell culture dishes (Greiner, Oberösterreich, Germany)
Glass coverslips (Matsunami Glass, Osaka, Japan)
DMEM/F-12 (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS (Thermo Fisher Scientific, Waltham, MA, USA) and PenStrep (Thermo Fisher Scientific)
5% CO2 at 37 °C

controls

Positive control: Not mentioned
Negative control: Cell culture without ibuprofen

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