Comprehensive Western Blotting Protocol

Western Blotting was performed as described [55 (link)]. The antibodies used and respective catalog numbers were as follows: anti-IKKβ (05-535), anti-IKKα (05-536) and anti-pan-RAS (OP-40) were from EMD Millipore (Billerica, MA); anti-IκBα (4812) and anti-phospho(s32/36)IκBα (9246) were from Cell Signaling (Danvers, MA); we also used anti-GAPDH (sc-25778) and anti-p53 antibodies (cs-2527, Cell Signaling, Danvers, MA and sc-98, Santa Cruz Biotechnology, Santa Cruz, CA).

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Bassères D.S., Ebbs A., Cogswell P.C, & Baldwin A.S. (2014). IKK is a therapeutic target in KRAS-Induced lung cancer with disrupted p53 activity. Genes & Cancer, 5(1-2), 41-55.

Publication 2014

anti-IκBα (4812) sc-98,

Anti antibodies Antibodies Gapdh Ikkα Ikkβ Iκbα

Corresponding Organization :

Other organizations : Universidade de São Paulo, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of IKKβ, IKKα, pan-RAS, IκBα, and phospho(s32/36)IκBα proteins

control variables

GAPDH and p53 protein levels (used as loading controls)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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