Transfection of Colon Cell Lines

Human colon epithelial CCD 841 CoN (CRL-1790, ATCC, Manassas, VA, USA) and colorectal adenocarcinoma SW480 (CCL-228, ATCC, Manassas, VA, USA) and SW620 (CCL-227, ATCC, Manassas, VA, USA) cells were grown as previously reported [39 (link)]. Cells were transfected for 6 h in serum- and antibiotic-free medium with 40 nM miRNA mimic Negative Control (miR-NC, MCH00000), hsa-miR-148a miRNA mimic (miR-148a⁺, MCH01336), hsa-miR-148a miRNA agomir (a-miR-148a, MAH01336), or miRNA agomir Negative Control (a-miR-NC, MAH00000), all from Applied Biological Materials, Inc., Richmond, BC, Canada, before the addition of fetal bovine serum (FBS). Lullaby (LL70500, OZ Biosciences, Marseille, France) was used for transfection following the manufacturer’s instructions. The cells were incubated up to 72 h after transfection.