Live/Dead Cell Imaging Assay

Cells were seeded in a 24-well plate overnight. After treatment, cells were stained with the LIVE/DEAD Cell Imaging kit (488/570) (Thermo Fisher Scientific, Inc., Waltham, MA) as described previously [49 (link)]. After incubation at room temperature in the dark, the cells were observed under EVOS FLoid Cell Imaging System (Thermo Fisher Scientific) and measured for live cells (green) with Green-Light Channel and dead cells (red) with Red-Light Channel.

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Lyu H., Shen F., Ruan S., Tan C., Zhou J., Thor A.D, & Liu B. (2023). HER3 functions as an effective therapeutic target in triple negative breast cancer to potentiate the antitumor activity of gefitinib and paclitaxel. Cancer Cell International, 23, 204.

Publication 2023

LIVE/DEAD Cell Imaging kit (488/570) EVOS FLoid Cell Imaging System

Cells Light

Corresponding Organization :

Other organizations : Louisiana State University Health Sciences Center New Orleans, First Affiliated Hospital of Soochow University, Soochow University, Nanjing Medical University, University of Colorado Anschutz Medical Campus

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Variable analysis

independent variables

Treatment

dependent variables

Number of live cells (green fluorescence)
Number of dead cells (red fluorescence)

control variables

Cell seeding density
Incubation time
Staining protocol

controls

Positive control: Untreated cells
Negative control: Not specified

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