Single Cell Cycle Analysis
Corresponding Organization :
Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Sun Yat-sen University, Shanghai Medical College of Fudan University, Shanghai Institute of Organic Chemistry
Variable analysis
- Isolation and suspension of single cells from kidney cortex tissue
- Cell cycle distribution
- Fixation of single cell suspension in 1% paraformaldehyde at 4 °C overnight
- Incubation of single cell suspension with propidium iodide/RNase staining solution for 20 min
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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