Structural Modeling of Mic60 and Mic19

The model of Mic60 and Mic19 in CJs was created and prepared by E. Werner, RNS Berlin (www.rns.berlin), who granted copyrights. The model comprises the crystal structures of the CC region of Mic60 from L. thermotolerans (ltMic60_CC, residues 235 to 382), the LBS 1 and mitofilin domain of ctMic60, and the CHCH domain of ctMic19 (Mito2_CHCH). In this, the sequence of ltMic60 was used as template. All other parts of Mic60 were modeled as unstructured regions. A CJ diameter of 17.5 nm was used (13 (link)). The model was generated using the Maya software from Autodesk Inc. (www.autodesk.com/products/maya/) and Modeling kit and Rigging kit of the plugin Molecular Maya (mMaya) from Digizyme Inc. (https://clarafi.com/tools/mmaya/).

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Bock-Bierbaum T., Funck K., Wollweber F., Lisicki E., von der Malsburg K., von der Malsburg A., Laborenz J., Noel J.K., Hessenberger M., Jungbluth S., Bernert C., Kunz S., Riedel D., Lilie H., Jakobs S., van der Laan M, & Daumke O. (2022). Structural insights into crista junction formation by the Mic60-Mic19 complex. Science Advances, 8(35), eabo4946.

Publication 2022

Maya software

Corresponding Organization : Saarland University

Other organizations : Max Planck Institute for Biophysical Chemistry, Martin Luther University Halle-Wittenberg, Max Planck Institute for Dynamics and Self-Organization, Universitätsmedizin Göttingen, Fraunhofer Institute for Translational Medicine and Pharmacology

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Variable analysis

independent variables

Model of Mic60 and Mic19 in CJs

dependent variables

Crystal structures of the CC region of Mic60 from L. thermotolerans (ltMic60CC, residues 235 to 382)
LBS 1 and mitofilin domain of ctMic60
CHCH domain of ctMic19 (Mito2_CHCH)

control variables

CJ diameter of 17.5 nm

controls

Not explicitly mentioned

Annotations

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