The human urothelial carcinoma cell line EJ28Luc, isolated from a primary bladder carcinoma, was grown in RPMI medium supplemented with 10% fetal calf serum and 1% non-essential amino acids (Biochrom, Berlin, Germany) in a humified atmosphere containing 5% CO2 at 37 °C. Transfection of cells was previously carried out with plasmid pcDNA3.1 containing the coding sequence of firefly (Photinus pyralis) luciferase under the control of the cytomegalovirus promoter12 (link). The human glioma cell line LN18 was cultured in RPMI medium supplemented with 10% fetal calf serum. Cells were harvested after rinsing the monolayer with an EDTA/PBS solution (1 mM EDTA in PBS; Biochrom) for 10 min at 37 °C, respectively.
EJ28Luc cells were a gift from Birgit Pfost. LN18 cells were gifted from Jürgen Schlegels’ lab. All methods were carried out in accordance with relevant guidelines and regulations.
Both cell lines were chosen based on the high EGFR expression as deduced from binding of 213Bi-anti-EGFR-MAb > 60%, allowing for a targeted treatment. Binding of 213Bi-anti-EGFR to both cell lines was shown previously15 (link).
EJ28Luc cells were a gift from Birgit Pfost. LN18 cells were gifted from Jürgen Schlegels’ lab. All methods were carried out in accordance with relevant guidelines and regulations.
Both cell lines were chosen based on the high EGFR expression as deduced from binding of 213Bi-anti-EGFR-MAb > 60%, allowing for a targeted treatment. Binding of 213Bi-anti-EGFR to both cell lines was shown previously15 (link).
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