The rabbit polyclonal antibodies recognizing the C terminus of IBV E and the N-terminal head of golgin-160 have been previously described (42 (link), 43 (link)). The antibody generated against the C terminus of IBV S (anti-IBV SCT) has also been previously described (26 (link)). The mouse monoclonal antibody recognizing IBV S1 was a gift from Ellen Collisson (25 (link)). The mouse monoclonal antibody recognizing the N terminus of influenza A virus M2 has been previously described and was a generous gift from Andrew Pekosz (44 (link)). The mouse anti-GFP antibody was obtained from Roche (Mannheim, Germany). The rabbit anti-GFP antibody was obtained from Thermo Fisher Scientific (Rockford, IL). Alexa Fluor 488-conjugated anti-rabbit IgG, Alexa Fluor 488-conjugated anti-mouse IgG, Alexa Fluor 568-conjugated anti-rabbit IgG, and Alexa Fluor 568-conjugated anti-mouse IgG were obtained from Invitrogen/Molecular Probes (Eugene, OR).
Protocol detail
Antibody Characterization for Viral Protein Detection
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mouse anti-GFP antibody
rabbit anti-GFP antibody
Alexa Fluor 488-conjugated anti-rabbit IgG
Alexa Fluor 488-conjugated anti-mouse IgG
Alexa Fluor 568-conjugated anti-mouse IgG
Alexa 568
Alexa fluor 488
Anti antibody
Anti igg
Antibodies
Antibody
Golgin
Head
Influenza a virus
Molecular probes
Monoclonal antibody
Mouse
Rabbit
Corresponding Organization :
Other organizations : Johns Hopkins Medicine, Johns Hopkins University
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Variable analysis
independent variables
- Rabbit polyclonal antibodies recognizing the C terminus of IBV E
- Rabbit polyclonal antibodies recognizing the N-terminal head of golgin-160
- Antibody generated against the C terminus of IBV S (anti-IBV S_CT)
- Mouse monoclonal antibody recognizing IBV S1
- Mouse monoclonal antibody recognizing the N terminus of influenza A virus M2
- Mouse anti-GFP antibody
- Rabbit anti-GFP antibody
- Alexa Fluor 488-conjugated anti-rabbit IgG
- Alexa Fluor 488-conjugated anti-mouse IgG
- Alexa Fluor 568-conjugated anti-rabbit IgG
- Alexa Fluor 568-conjugated anti-mouse IgG
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: None specified
- Negative control: None specified
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