Human Huh7 (Health Science Research Resources Bank JCRB0403), mouse Hepa-1 (ATCC CRL-1830) and human 293T (ATCC CRL-3216) were maintained as described previously (37 (link), 41 (link)). For ACD treatment, exponentially growing cells were treated with 500 ng/ml ACD for 0, 30 and 60 min. Standard methods were used for RNA and protein isolation, cDNA synthesis, qPCR, WB, transient transfection, and luciferase reporter assay (42 (link), 43 (link)). Experiments were done in triplicate from three independent experiments.
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ACD Treatment Induces Transcriptional Changes
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Corresponding Organization : Wenzhou Medical University
Other organizations : Université Laval
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Variable analysis
independent variables
- ACD treatment
- RNA and protein expression
- CDNA synthesis
- WB (Western Blot)
- Transient transfection
- Luciferase reporter assay
- Cell lines (Human Huh7, mouse Hepa-1, human 293T)
- Cell culture conditions (as described previously)
- Positive controls: Not explicitly mentioned.
- Negative controls: Not explicitly mentioned.
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