Trypanocidal Assay of Compounds

The extract and the purified compounds were cultured and tested against T. b. brucei, and T. congolense exactly as described previously, using a resazurin-based assay [16 (link),17 (link)]. The T. b. brucei strains were a standard drug-sensitive lab strain, Lister 427 (wild-type) [47 (link)] and the derived cell line B48 was developed from the wild-type by gene deletion of the drug transporter TbAT1 followed by in vitro adaptation to pentamidine [48 (link)], leading to the further loss of the gene encoding TbAQP2 [49 (link)], rendering it highly resistant to the diamidine and melaminophenyl arsenical classes of trypanocides. The T. congolense strains were the lab strain IL3000 and its diminazene-adapted clone 6C3 [50 (link)]. All EC₅₀ values presented are the average of at least three independent determinations.

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Alenezi S.S., Alenezi N.D., Ebiloma G.U., Natto M.J., Ungogo M.A., Igoli J.O., Ferro V.A., Gray A.I., Fearnley J., de Koning H.P, & Watson D.G. (2023). The Activity of Red Nigerian Propolis and Some of Its Components against Trypanosoma brucei and Trypanosoma congolense. Molecules, 28(2), 622.

Publication 2023

Arsenical Assay Cell line Clone Diamidine Diminazene Drug Gene Gene deletion Pentamidine Resazurin Strain Transporter Trypanocides

Corresponding Organization : University of Glasgow

Other organizations : Teesside University, Ahmadu Bello University, Gombe State University, Whitby Hospital

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Variable analysis

independent variables

Extracts and purified compounds

dependent variables

Antitrypanosomal activity against T. b. brucei and T. congolense

control variables

T. b. brucei strains (Lister 427 wild-type and B48 derived cell line)
T. congolense strains (IL3000 and diminazene-adapted clone 6C3)
Resazurin-based assay method

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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