Genomic DNA from P. savastanoi strain MHT1 was extracted with the TIANamp Bacteria DNA Kit (TIANGEN BIOTECH, Beijing, China) and quality-tested on a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). DNA concentration was measured on a Nanodrop (Thermo Fisher Scientific, Wilmington, USA).
Genome sequencing was performed on a PacBio long-read sequencer (Pacific Biosciences, Menlo Park, CA, USA). SMRTbell libraries were obtained using g-TUBE (Covaris, Woburn, Massachusetts, USA) and end repair. According to the manufacturer’s protocols of the Blue Pippin system (Pacific Biosciences), fragments with sizes larger than 10 kb were selected. The quality and average size of the fragments of the library were estimated using a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The PacBio Sequel system (Pacific Biosciences, Menlo Park, CA, USA) was used to perform SMRT sequencing. The resulting continuous long reads were used for de novo assembly with the Falcon program (version 0.3.0) [36 (link)].
Genome sequencing was performed on a PacBio long-read sequencer (Pacific Biosciences, Menlo Park, CA, USA). SMRTbell libraries were obtained using g-TUBE (Covaris, Woburn, Massachusetts, USA) and end repair. According to the manufacturer’s protocols of the Blue Pippin system (Pacific Biosciences), fragments with sizes larger than 10 kb were selected. The quality and average size of the fragments of the library were estimated using a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The PacBio Sequel system (Pacific Biosciences, Menlo Park, CA, USA) was used to perform SMRT sequencing. The resulting continuous long reads were used for de novo assembly with the Falcon program (version 0.3.0) [36 (link)].
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